Essay on Inoculation Techniques

The technique of aseptic removal and transfer of microorganisms for subculturing

HYPOTHESIS

Culture transfer technique is the best approach used to transfer and inoculate organisms.

EQUIPMENT

1. One nutrient broth

2. One nutrient agar slant

3. One nutrient agar deep tube

4. Bunsen burner

5. Inoculating loop and needle

6. Glassware marking pencil

PROCEDURE

1. Label the tube to be inoculated with the name of the organism and your initials in this manner:

a. Nutrient agar plates

b. Nutrient agar slants

c. Nutrient broth tube

2. Place the tube in the palm of your hand, secure with your thumb and separate to form a V.

3. Uncap the agar slant and pass the neck of the tube rapidly over the Bunsen burner flame.

4. Inoculate the slant by drawing the needle upward in a zigzag motion along the surface of the agar. Do not dig into the agar.

5. Flame the neck of the tube and recap

6. Flame the inoculating needle

7. Incubate all cultures at 37°C for 24 to 48 hours.

OBSERVATIONS AND RESULTS

The nutrient broth tube was positive for growth. The growth was slight in both locations and was pink in color. However, the broth remained yellow and transparent for most of the volume of the tube.The nutrient agar slant tube was positive for growth. Growth was bound to the slant surface and was slight. The growth displayed an orange-red coloration. The remaining slant displayed the characteristics of yellow color and was clear.

CONCLUSION

Both tubes exhibited growth. With that, it can be deduced that the proper techniques were used, giving successful in the transfer and inoculation of both tubes with the organism.