Green Tea and Inflammatory Breast Cancer

The research was carried out to investigate whether an element is green tea polyphenol epigallocatechin-3-gallate could be used to treat inflammatory breast cancer which does not a specific treatment. This form of cancer is very dangerous and can results in death, therefore the need to investigate how it can be suppressed is very critical. Many people believe that green tea reduces the risk of cancer diseases, therefore the purpose of the research was to help find out whether polyphenol epigallocatchin-3-gallate which is an element in the green tea could offer a solution to inflammatory breast cancer (Mineva, Paulson, Naber, Yee,  & Sonenshein, 2013). In-order to carry out this investigation, the two cell cultures (SUM-149 and SUM-190) were treated with the polyphenol epigallocatechin-3-gallate (EGCG) and the results were monitored and interpreted.

In the Materials and Methods section of the paper, the authors discuss the cell culture and treatment conditions. What was the control group treated with in this section (make sure to look only at the Cell Culture and Treatment section of the paper)? Why?

The cancer cells containing SUM-149 and SUM 190 cells were extracted from animal and placed in a culture then treated with polyphenol epigallocatechin-3-gallate to analyze whether this would help to suppress the cell from multiplying in culture. The two cells SUM-149 and 190 were extracted and kept in an environment that can make them survive. Upon treating the cells with a solution containing 5 percent fetal bovine serum, insulin, and penicillin, the cells froze and the new culture was introduced.  Testing carried using the mycoplasma kit indicated that the cells did not have mycoplasma meaning solution had reduced the mycoplasma contents. The higher concentration of polyphenol epigallocatechin-3-gallate when reacted with the cell reduced the number of the cells that were in the culture.  From the findings, these make polyphenol and epigallocatechin-3-gallate as the best solution for reducing inflammatory breast cancer.

Again, looking only at the Cell Culture and Treatment section, what was the experimental group treated with? Why?

            The cancer cell SUM-149 is introduced in a buffer solution containing 1.5 moles per litre bodipyaminoacetadehyde substrate, which then creates environments that favour cell activity. Inflammatory breast cancer cells were treated with polyphenol epigallocatechin-3-gallate which destroy the gene that encourages the growth of the cancer cell. Tumor formation due to the cancer cells was greatly reduced through the introduction of the epigallocatechin-3-gallate compound.

In the Results section of the paper, the authors clearly summary their multiple findings. In the "EGCG Reduces Growth of Pre-existing Tumors Derived from SUM-149 Stem-like Cells" results sub-section, the authors report a specific finding. What specific quantitative results do the researchers report?

The quantitative results involve the use of the quantified volume of polyphenol and epigallocatechin-3-gallate in carrying out the analysis. From the results, it is indicated that epigallocatechin-3-gallate reduced the effects of inflammatory breast cancer cells to almost 50 percent (Mineva, Paulson, Naber, Yee, & Sonenshein, 2013). Based on the experiments, the data is quantified to GAPDH control and presented in the sum of averages for easier interpretation. The graphical presentation is used to indicate how the how different concentration of epigallocatechin-3-gallate and the percentage of the cell.

Did the researchers follow the scientific method in their experimental design? Explain step by step specifically what did they do that shows whether or not they satisfied every step of the scientific method.

Scientific methods were used by the researchers, owing to the facts that experiments were carried out to show the significance of different concentration of epigallocatechin-3-gallate used in the research. First, isolation of the cells from the rabbit for was done through surgery, then scientific methods are used to keep the cells in culture.

Based on the results, was the hypothesis supported, explain your answer, and what can you conclude from this experiment?

Based on the research, one can deduce that green tea polyphenol epigallocatechin-3-gallate can control inflammatory breast cancer cells owing to the facts that it decreases proliferation of the cells. Polyphenol epigallocatechin-3-gallate decreases the growth of cancers cells, therefore, making a vital tool for suppression of inflammatory breast cancer. The findings proved that green tea can effectively be used to reduce the cancer ailments, therefore, patients ought to be encouraged to use green tea frequently. An increase in the concentration or dozes of epigallocatechin-3-gallate will reduce the cancer cells from multiplying within the body.

References

Mineva, N. D., Paulson, K. E., Naber, S. P., Yee, A. S., & Sonenshein, G. E. (2013). Epigallocatechin-3-gallate inhibits stem-like inflammatory breast cancer cells. PloS one, 8(9), e73464.